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发布于:2022-1-9 06:28:45  访问:59 次 回复:0 篇
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Sed. To Your Greatest Of Our Understanding, This Is Actually The Very First
Sed. For the most effective of our knowledge, here is the 1st study to employ non-targeted techniques to recognize candidate genes in peach. The established of genes linked to aroma compounds provided herein could confirm helpful for marker-assisted breeding and/or biotechnology improvements of peach fruit.MethodsFruit product and analysisPeach fruit (genotypes `MxR_01` and `Granada`) ended up harvested in July 2009 from the community industrial orchard situated in Murcia, Spain. `MxR_01` is actually a freestone melting-flesh peach which was acquired with the IVIA (Instituto Valenciano de Investigaciones Agrarias) breeding program. `MxR_01` is really a seedling from the cross amongst `RedCandem` and `Maruja`, a standard Spanish germplasm. `Granada` is really a clingstone non-melting peach small chilling acquired from the Brazilian breeding plan. Fruits equivalent to 4 maturity levels (S1, S2, S3, and S4) were being harvested from one tree per genotype. For S1, S2, and S3, 10 fruits from just about every maturity phase were gathered and maturity parameters were right away analyzed. For S4, "harvest ripe" (or ready to obtain) phase, 20 fruits ended up harvested and divided into two teams. One particular team (denominated S4) was straight away analyzed plus the other (denominated S4+SL) was subjected to shelf-life ailments (2 times at 20 , 85 RH) ahead of the maturity examination. S4+SL corresponded to "consumption ripe" (or "ready to eat") phase. The maturity parameters (external color, flesh firmness, bodyweight, total soluble solids (SSC), and ethylene and CO2 manufacturing) had been analyzed as explained in Sanchez et al. [11]. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/15645520 A Principal Parts chez et al. BMC Genomics 2013, fourteen:343 http://www.biomedcentral.com/1471-2164/14/Page three ofAnalysis was performed with maturity parameters if you want to discover the a few most homogeneous fruits per maturity stage for every genotype (data not demonstrated). These a few fruits (organic replicates) were chosen for risky and microarray analyses.Sample planning and HS-SPME-GC-MS conditionsFrozen samples of fruit mesocarp have been floor to powder in liquid nitrogen and useful for volatile and microarray analyses as follows. Risky compounds were being analyzed from five hundred mg of frozen tissue powder, as beforehand explained [11]. The volatile investigation was performed on an Agilent 6890N gas chromatograph coupled into a 5975B Inert XL MSD mass spectrometer (Agilent Systems). For the chromatography and HKOH-1r mass spectra situations, see S chez et al. [11]. A total of 36 industrial specifications were accustomed to confirm compound annotation; the VOCs verified are detailed in Added file 1: Figure S1. Volatiles were quantified rather through the Multivariate Mass Spectra Reconstruction (MMSR) technique designed by Tikunov et al. [28]. A detailed description on the quantification method is offered in S chez et al. [11].RNA extractionTelechem Hybridization Chambers (Corning), next the manufacturer`s directions. Following hybridization, slides were washed in 2x SSC, 0.1 SDS for five min at 42 , 0.1x SSC, 0.1 SDS for 10 min at home temperature, 0.1x SSC for five min at home temperature four instances, and 0.01x SSC for five min at room temperature 4 situations. Arrays have been drained by centrifugation at 528g for 2 min. Slides had been scanned having a GenePix 4000B scanner (Axon Instruments) at 10 m resolution, 100 laser energy and with distinctive PMT values to regulate the ratio to 1.0. Microarray visuals had been analyzed and globally normalized using the GenePix four.one program (Axon Instruments). Just the spots by using a backgroundsu.
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